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Updated: May 21, 2026

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
Published on: February 21, 2015
[Analysis of de novo copy number variations in a family affected with autism spectrum disorders using high-resolution
Wen-zhi He1, Wei-qiang Liu, Xin-qi Zhong
1Experimental Department of Institute of Obstetrics and Gynecology, the Third Affiliated Hospital of Guangzhou Medical University, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Guangzhou Key Laboratory of Reproductive Medicine and Genetics, Guangzhou, Guangdong 510150, P.R. China.
Objective:
To analyze de novo copy number variations (CNVs) in a Chinese family affected with autism spectrum disorders (ASD).
Methods:
Affymetrix Cytogenetics Whole Genome 2.7M Array assay was performed to identify potential CNVs in four members from the family.
Results:
A total of 89 de novo CNV regions were identified in the autistic siblings. The CNV regions in total have exceeded 1/1000 of the lengths of chromosomes 5, 11 and 14. In addition, de novo CNV regions were also identified at 3p26.1, 4q22.2, and 5p15.2, which encompassed 10 genes associated with nerve development including GRM7, GRID2 and CTNND2.
Conclusion:
A number of nerve development associated genes were at the de novo CNV sites, which may provide new clues for genetic research of ASD. High-resolution array-comparative genomic hybridization is an effective method for detecting submicroscopic chromosomal imbalances.
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