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Related Concept Videos

Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
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Insertion of Multi-pass Transmembrane Proteins in the RER

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The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
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Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
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Lipid Catabolism01:25

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Related Experiment Video

Updated: May 21, 2026

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

New molecular insights into CETP structure and function: a review.

M Arthur Charles1, John P Kane

  • 1Department of Medicine University of California, San Francisco, CA 94158, USA. art.charles@ucsf.edu

Journal of Lipid Research
|June 9, 2012
PubMed
Summary

Cholesteryl ester transfer protein (CETP) is a drug development target. New research clarifies its molecular mechanism and interactions with lipoproteins, improving understanding of lipid transfer.

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Cholesterol Efflux Assay
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Last Updated: May 21, 2026

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
09:37

Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Published on: August 15, 2014

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
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Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Cholesteryl ester transfer protein (CETP) plays a crucial role in lipid metabolism.
  • CETP is a significant target for novel drug development.
  • The precise structure and mechanism of CETP action remain incompletely understood.

Purpose of the Study:

  • To elucidate the molecular mechanism of CETP function.
  • To understand CETP's interactions with lipoproteins at a molecular level.
  • To provide insights for the development of CETP-targeted therapeutics.

Main Methods:

  • Integration of novel structural and functional methodologies.
  • Comparative analysis with existing experimental data.
  • Molecular-level functional step analysis.

Main Results:

  • Proposed a multi-step model for CETP function: sensing, penetration, docking, selectivity, ternary complex formation, lipid transfer, and HDL dissociation.
  • Detailed the molecular interactions between CETP and lipoproteins.
  • Provided new structural and functional insights into CETP activity.

Conclusions:

  • The study enhances the understanding of CETP's mechanism of action.
  • These findings offer a foundation for rational drug design targeting CETP.
  • Improved comprehension of CETP's role in lipid transfer and metabolism.