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Related Concept Videos

Bacterial Phylum Spirochaetes01:30

Bacterial Phylum Spirochaetes

Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased by a...

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Correction: Medkour et al. Adenovirus Infections in African Humans and Wild Non-Human Primates: Great Diversity and Cross-Species Transmission. <i>Viruses</i> 2020, <i>12</i>, 657.

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Multispacer sequence typing relapsing fever Borreliae in Africa.

Haitham Elbir1, Elbir Haitham, Gregory Gimenez

  • 1Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 6236 IRD 198, Faculté de Médecine, Méditerranée Infection, Aix-Marseille Université, Marseille, France.

Plos Neglected Tropical Diseases
|June 9, 2012
PubMed
Summary

A new multispacer sequence typing (MST) method effectively genotypes African relapsing fever Borrelia species. This tool aids in understanding the epidemiology and reservoirs of these neglected arthropod-borne infections.

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Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • Relapsing fevers in Africa are neglected arthropod-borne infections caused by Borrelia species.
  • These infections cause undifferentiated fever, posing significant risks, especially to pregnant women.
  • Limited genotyping tools hinder understanding of Borrelia reservoirs and epidemiology.

Purpose of the Study:

  • To develop and evaluate a novel genotyping tool for African relapsing fever Borrelia species.
  • To improve the understanding of Borrelia epidemiology and reservoirs in Africa.

Main Methods:

  • Genome sequence analysis identified 5 intergenic spacers from 10 chromosomal genes.
  • These spacers were incorporated into a multispacer sequence typing (MST) approach.
  • MST was applied to human blood specimens infected with Borrelia recurrentis, Borrelia duttonii, and Borrelia crocidurae.

Main Results:

  • The MST approach successfully resolved 60 strains and 3 type strains into 13 species-specific spacer types.
  • Borrelia crocidurae yielded 8 spacer types, Borrelia duttonii 3, and Borrelia recurrentis 2.
  • Phylogenetic analysis suggested these species are variants of a single ancestral Borrelia species.

Conclusions:

  • Multispacer sequence typing (MST) is a suitable method for identifying and genotyping African relapsing fever borreliae.
  • The developed MST approach can be applied to both clinical specimens and vectors.
  • This advancement facilitates research into the epidemiology and reservoirs of these neglected infections.