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Updated: May 21, 2026
A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium
Published on: May 31, 2024
[Experimental study on the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats]
Ling Lu1, Lian-Qun Jia, Wei Ma
1RInstitute of Chinese Medical Literature, Liaoning University of Traditional Chinese Medicine, Shenyang 110847. swayling@sohu.com
Objective:
To identify the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats using proteomic approach.
Methods:
Thirty-seven rats were randomly divided into 3 groups, i. e., the normal control group (Group 1), the Pi-qi deficiency syndrome model group (Group 2), and the reserpine group (Group 3). The Pi-qi deficiency syndrome model was established using excessive exerting combined with irregular diet, and peritoneally injecting Reserpine Injection (1 mg/mL) respectively. The ileum tissues were separated after identified fuzzy method. The differentially expressed proteins were separated with two dimensional electrophoresis (2-DE), analyzed by Mass Spectrometry, and identified by MASCOT Software.
Results:
Three proteins were differentially expressed in Group 2 and nine proteins were differentially expressed in Group 3 (P < 0.05).
Conclusion:
Pi-qi deficiency syndrome was closely related with decreased expression of albumin as well as increased expressions of trypsin and glucose-regulated protein 78.
