Duration of microbead seeding on endothelial cells significantly affects their response to magnetic excitation

Our investigation of endothelial cell rheology using optical magnetic twisting cytometry revealed that with time following incubation of ferromagnetic beads on the cells, beads were sinking into the cells and an increasing number of beads demonstrated apparent absurd negative rheological properties. In parallel, the beads' average rheological response changed considerably over time, both in magnitude and in distribution. It was hypothesized that the apparent negative rheological response was related to the above sinking process of seeded beads into the cells, resulting in an elevation of the beads' rotation axis, thus causing a reversal of the beads' lateral movement direction in response to twisting external magnetic excitation. The results suggest that microbead-based rheological characterization of cells should be interpreted with caution, while considering the time of data acquisition.