Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laryngeal Mask Airway: An Alternative for Neonatal Resuscitation in a US Safety-Net Hospital.

Respiratory care·2026
Same author

Serotonin-deficient neonatal mice are not protected against the development of experimental bronchopulmonary dysplasia or pulmonary hypertension.

Physiological reports·2022
Same author

Utilization of Laboratory Testing Algorithms for Celiac Disease in a Pediatric Hospital.

Laboratory medicine·2019
Same author

Acceptability of self-sample human papillomavirus testing among medically underserved women visiting the emergency department.

Gynecologic oncology·2015
Same author

Activating B cell signaling with defined multivalent ligands.

ACS chemical biology·2007
Same author

The role of helix stabilizing residues in GCN4 basic region folding and DNA binding.

Protein science : a publication of the Protein Society·2002
Same journal

Toward Sustainable Optics: Fully Biomass-Based Vitrimer Film for Near-Infrared Transparency.

Biomacromolecules·2026
Same journal

Poly(furfuryl alcohol) as a Surface Modifier for Cellulose Nanocrystals Reinforced HDPE Nanocomposites.

Biomacromolecules·2026
Same journal

Cutinase Adsorption to Polyester Surfaces Modulates Dissolved Oligomer Speciation during Enzymatic Depolymerization.

Biomacromolecules·2026
Same journal

Controlled Synthesis of Sulfated Glycopolypeptides as Heparin Mimics via <i>N</i>-Carboxyanhydride ROP for Anticoagulant Property.

Biomacromolecules·2026
Same journal

Ligno-Suberized Fruit Periderm as a Mechanically Reinforced Biomacromolecular Composite.

Biomacromolecules·2026
Same journal

Effect of Hydrophilic Brush Length and Hydrophobic Chain on Biodistribution of Polymethacrylate-Based Statistical Copolymers.

Biomacromolecules·2026
See all related articles

Related Experiment Video

Updated: May 21, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Designed ankyrin repeat proteins as scaffolds for multivalent recognition.

Jessica J Hollenbeck1, Derek J Danner, Rachel M Landgren

  • 1Department of Chemistry, Trinity University, San Antonio, Texas 78212, United States. jessica.hollenbeck@trinity.edu

Biomacromolecules
|June 12, 2012
PubMed
Summary
This summary is machine-generated.

Ankyrin repeat (AR) proteins can be engineered into novel multivalent ligands. These glycosylated AR scaffolds efficiently cluster concanavalin A (Con A), demonstrating their potential in studying molecular interactions.

More Related Videos

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
08:02

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Published on: June 27, 2016

Related Experiment Videos

Last Updated: May 21, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
08:02

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Published on: June 27, 2016

Area of Science:

  • Biochemistry
  • Structural Biology
  • Biotechnology

Background:

  • Ankyrin repeat (AR) proteins possess a modular structure, ideal for presenting multiple functional groups.
  • This modularity allows for the creation of well-defined, chemically homogeneous multivalent ligands.
  • Understanding multivalent interactions is crucial in various biological and biotechnological applications.

Purpose of the Study:

  • To develop a biosynthetic strategy for generating novel multivalent ligand scaffolds using AR proteins.
  • To investigate the efficacy of these engineered AR proteins in clustering specific molecular targets.

Main Methods:

  • Utilized the modular nature of AR proteins for a biosynthetic strategy.
  • Generated chemically homogeneous and structurally defined multivalent ligands.
  • Assessed the clustering of tetrameric concanavalin A (Con A) by glycosylated AR proteins.

Main Results:

  • Engineered AR proteins effectively formed multivalent ligands.
  • Glycosylated AR proteins demonstrated efficient clustering of concanavalin A (Con A).
  • The clustering rate was comparable to existing multivalent ligand systems.

Conclusions:

  • AR proteins represent a new class of versatile multivalent ligand scaffolds.
  • These scaffolds have significant potential for studying and controlling multivalent interactions.
  • The findings open avenues for applications in drug delivery, diagnostics, and biomaterials.