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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 21, 2026

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

Dissecting immunity by germline mutagenesis.

Onjee Choi1, Sophie Rutschmann

  • 1National Heart and Lung Institute, Imperial College London, London, UK.

Immunology
|June 12, 2012
PubMed
Summary

Germline mutagenesis in mice is a powerful forward genetics tool for understanding immune system pathways. This method has identified novel genes and protein functions crucial for innate and adaptive immunity, aiding in deciphering immune deficiencies.

Area of Science:

  • Immunology
  • Genetics
  • Molecular Biology

Background:

  • Understanding immune system regulation is crucial for addressing immune deficiencies.
  • Current genetic and technological approaches offer incomplete insights into immune pathways.
  • In vivo strategies are essential for further elucidating pathogen detection and eradication mechanisms.

Purpose of the Study:

  • To review germline mutagenesis in mice as a forward genetic approach.
  • To highlight the role of specific mouse mutants in understanding immune responses.
  • To showcase how this method identifies new genes and functions in immunity.

Main Methods:

  • Focuses on germline mutagenesis in mice as a forward genetic strategy.
  • Describes technical aspects of generating and analyzing germline mutants.

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Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

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Related Experiment Videos

Last Updated: May 21, 2026

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches

Published on: April 20, 2021

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
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Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Published on: November 1, 2011

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency
14:45

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

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  • Discusses specific examples of mouse mutants and their impact on immunological research.

    • Main Results:

    • Germline mutagenesis has identified previously uncharacterized genes (e.g., Unc93B, Themis) critical for immunity.
    • Mutants have elucidated functions of specific protein domains (e.g., PanR1).
    • This approach revealed novel gene functions, such as memi's link to cell proliferation and apoptosis.

    Conclusions:

    • Germline mutagenesis is an effective non-hypothesis-driven method for discovering new players in immune responses.
    • This technique provides valuable insights into both innate and adaptive immunity.
    • Identifying genetic causes of immune deficiencies can be advanced through this strategy.