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Updated: May 21, 2026

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
Compositional equivalence of barleys differing only in low- and normal-phytate levels
Robert A Moreau1, Phil Bregitzer, KeShun Liu
1Sustainable Biofuels and Coproducts Research Unit, Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, Pennsylvania 19038, United States. robert.moreau@ars.usda.gov
Breeding barley for lower phytate (a phosphorus inhibitor) did not alter other key nutritional components. This finding confirms that low-phytate barley retains its overall nutritional value for animal feed.
Area of Science:
- Agricultural Science
- Nutritional Biochemistry
- Plant Breeding
Background:
- Phytate (phytate P) in barley grain reduces phosphorus and mineral bioavailability in animal diets.
- Recent breeding efforts have produced barley lines with significantly reduced phytate levels (≥50%).
- Low-phytate (LP) barley offers enhanced P and mineral cation bioavailability.
Purpose of the Study:
- To investigate if breeding for reduced phytate content affects other nutritional characteristics in barley.
- To compare key nutritional attributes between low-phytate and normal-phytate barley cultivars.
Main Methods:
- Comparative analysis of five LP and five normal-phytate barley lines.
- Quantification of protein, oil, ash, total carbohydrate, starch, and β-glucan.
- Analysis of fatty acid composition, tocopherols, and tocotrienols.
- Barley samples were grown across three locations in Idaho.
Main Results:
- Only phytate levels were significantly lower in the LP barley group compared to the normal-phytate group.
- All other measured nutritional attributes, including protein, oil, starch, and β-glucan, were equivalent between the two groups.
- Fatty acid profiles, tocopherols, and tocotrienols remained consistent across both barley types.
Conclusions:
- Breeding barley for reduced phytate content does not negatively impact other major nutritional components.
- LP barley maintains comparable levels of protein, oil, carbohydrates, and micronutrients.
- This suggests that LP barley is a viable option for improving animal nutrition without compromising overall seed quality.
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