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Related Experiment Video

Updated: May 21, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

Cryptococcus spp isolated from dust microhabitat in Brazilian libraries.

Diniz P Leite¹, Janaina V R S Amadio, Evelin R Martins

¹Medicine Faculty, Mycology laboratory, Federal University of Mato Grosso, Fernando Correa da Costa Avenue, 2367, Boa Esperança - Cuiabá/MT, Brazil. rchahn@terra.com.br.

Journal of Occupational Medicine and Toxicology (London, England)

|June 12, 2012

Summary

Cryptococcus yeasts were found in 21.4% of book dust samples in Brazilian libraries. This highlights potential exposure to fungal pathogens like Cryptococcus gattii and Cryptococcus neoformans in everyday environments.

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Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

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Last Updated: May 21, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

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Published on: May 2, 2018

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

Area of Science:

Medical Mycology
Environmental Microbiology

Background:

Cryptococcus species are encapsulated yeasts with a worldwide distribution, known to cause cryptococcosis.
These fungi are commonly found in animal and plant-derived materials, with human infection typically occurring via spore inhalation.

Purpose of the Study:

To investigate the presence and diversity of Cryptococcus species in book dust within urban library environments in Cuiabá, Mato Grosso, Brazil.
To assess the potential risk of cryptococcosis transmission from library dust.

Main Methods:

Eighty-four book dust samples were collected from three libraries.
Samples were cultured on Sabouraud agar and birdseed (Niger) agar at 37°C.
Identification involved India ink staining, L-canavanine glycine bromothymol blue (CGB) tests, urea broth, and carbohydrate assimilation tests.

Main Results:

Eighteen out of 84 samples (21.4%) tested positive for Cryptococcus spp., yielding 41 colony-forming units.
The most prevalent species identified was Cryptococcus gattii (36.6%), followed by Cryptococcus terreus (29.3%).
Other identified species included C. luteolus, C. neoformans, C. uniguttulatus, C. albidus, and C. humiculus.

Conclusions:

The high diversity of Cryptococcus yeasts in urban environmental sources, such as library book dust, indicates frequent exposure for individuals.
This study demonstrates the potential for acquiring cryptococcosis infection from environmental sources like library dust, posing a risk to both immunocompromised and healthy individuals.