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Striatum specific protein, Rhes regulates AKT pathway
Sookhee Bang1, Catherine Steenstra, Sangwon F Kim
1Department of Psychiatry and Pharmacology, Center for Neurobiology and Behavior, University of Pennsylvania School of Medicine, 125 S 31st St., TRL Rm 2207, Philadelphia, PA 19104, United States.
Neuroscience Letters
|June 12, 2012
Summary
The Rhes GTPase interacts with PI3K
Area of Science:
- Neuroscience
- Molecular Biology
- Biochemistry
Background:
- The Rhes/RASD2 GTPase complex is implicated in dopamine receptor signaling and behavior.
- Rhes is a member of the RAS superfamily, predominantly expressed in the striatum, and possesses a unique C-terminal tail.
- Dopamine depletion has been previously linked to reduced Rhes mRNA expression.
Purpose of the Study:
- To investigate the molecular interactions and signaling pathways involving the Rhes GTPase.
- To elucidate the role of Rhes in the striatum and its connection to the AKT pathway.
Main Methods:
- Investigated the interaction between Rhes and p85, the regulatory subunit of PI3K.
- Examined the effect of growth factor treatment on the Rhes-p85 interaction in vitro.
- Assessed the influence of Rhes and the Rhes-p85 complex on AKT translocation to the cell membrane.
Main Results:
- Rhes directly interacts with p85, the regulatory subunit of PI3K, via its unique C-terminal tail.
- This interaction is enhanced by growth factor treatment.
- Rhes, particularly in complex with p85, facilitates AKT translocation to the membrane.
Conclusions:
- Rhes is a novel regulator of the AKT-mediated pathway in the striatum.
- The interaction between Rhes and p85 is a key mechanism through which Rhes influences AKT signaling.
- These findings provide new insights into the molecular underpinnings of striatal function and dopamine signaling.
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