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Updated: May 21, 2026

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
Published on: September 21, 2017
Flow injection-chemiluminescence determination of dopamine using potassium permanganate and formaldehyde system
Saikh Mohammad Wabaidur1, Zeid Abdullah Alothman, Seikh Mafiz Alam
1Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia. tarabai22@gmail.com
Abstract:
A simple and sensitive flow injection-chemiluminescence method for the determination of dopamine has been proposed. The method is based on the enhancing effect of dopamine on the chemiluminescence emission generated by the reaction of potassium permanganate with formaldehyde in an acidic medium. The proposed procedure allows the determination of dopamine over the concentration range of 3.1×10(-8)-1.7×10(-5) mol/L and with a detection limit of 1.0×10(-8) mol/L. The linear regression equation was F=44.4912+1.07×10(9)(*)C (correlation coefficient, r(2)=0.9998). The relative standard deviation is 2.1% for the determination of 1.0×10(-8) mol/L dopamine (n=11). The method was successfully applied to the determination of dopamine in pharmaceutical preparation with satisfactory results. The recoveries were found in the range of 96.5-101.3%.
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