Related Experiment Video
Updated: May 21, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
Published on: November 1, 2024
Efficiency of heteronuclear dipolar decoupling schemes in solid-state NMR: investigation of effective transverse
Venus Singh Mithu1, Supriya Pratihar, Subhradip Paul
1Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India.
Abstract:
We here compare the T(2)(') values of various heteronuclear dipolar decoupling schemes commonly used in solid-state nuclear magnetic resonance experiments. Swept-frequency two-pulse phase modulation scheme is shown to give longer T(2)(') values for the majority of the magic-angle-spinning frequencies and radiofrequency amplitudes considered here. The longer T(2)(') values obtained are shown to yield spectra with higher resolution in experiments, such as INADEQUATE, which incorporate spin-echo blocks. Such blocks normally constitute the indirect dimension of a multidimensional experiment during which heteronuclear dipolar decoupling is applied, thereby making the relevance of T(2)(') manifest clearly. Experimental results are shown on samples of glycine, alanine, and Aβ(42).
Related Concept Videos
Atomic Nuclei: Types of Nuclear Relaxation
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers energy to a nearby...
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Two-Dimensional (2D) NMR: Overview
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
