Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces
Andrzej Lyskowski1, Martin Tengg, Georg Steinkellner
1AAustrian Centre of Industrial Biotechnology, GmbH, Petersgasse 14, 8010 Graz, Austria. andrzej.lyskowski@acib.at
Abstract:
Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.02, b = 82.87, c = 76.77 Å, β = 96.93°.
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