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Updated: Aug 14, 2026
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Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration
Published on: September 6, 2014
A rapid procedure for eliminating chromatofocusing buffer and concentrating minor active subforms of mitochondrial
J L Gelpí1, V Gracia, S Imperial
1Department de Bioqúimica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
Biotechniques
|November 1, 1990
Abstract:
Mitochondrial malate dehydrogenase from several sources contains different molecular forms whose origin is still under discussion. Separation of these subforms has been achieved by chromatofocusing. A simple and rapid method, based on 5' AMP Sepharose chromatography, has been developed to concentrate mitochondrial malate dehydrogenase subforms and simultaneously remove chromatofocusing buffer.
