Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disruption to TFEB signaling and autophagy in newly formed oligodendrocytes leads to aberrant generation of CNS myelin.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Selective enrichment of TCF4 in GABAergic neurons during postnatal primate development.

Frontiers in neuroanatomy·2026
Same author

Therapeutic targeting of fibrin-microglia interactions ameliorates Alzheimer's disease-related hyperexcitability and brain network dysfunction.

bioRxiv : the preprint server for biology·2026
Same author

Resilience to neuronal hyperactivity and restoration of the neuroimmune interactome by blocking fibrin-induced microglia activation in Alzheimers disease.

bioRxiv : the preprint server for biology·2026
Same author

A birth certificate for data to improve findability, accountability, and traceability.

NAR genomics and bioinformatics·2026
Same author

Adam9-deficient retinal pigment epithelium pseudopods maintain photoreceptor outer segment renewal despite subretinal space expansion.

The Journal of clinical investigation·2026
Same journal

Inhibitory Neurons in Human Anterior Entorhinal Cortex and Some Comparisons With the Rhesus Monkey.

The Journal of comparative neurology·2026
Same journal

In Situ Hybridization Chain Reaction and Immunohistochemical Labeling of the Octopamine Production Pathway in the Central Nervous System of Lymnaea stagnalis.

The Journal of comparative neurology·2026
Same journal

Innervation Pattern of Inhibitory Projection Neurons in the Bird Sound Localization Circuit.

The Journal of comparative neurology·2026
Same journal

Mu Opioid Receptor mRNA and Protein Localization Across the Rat and Mouse Habenula.

The Journal of comparative neurology·2026
Same journal

Proline-Rich Transmembrane Protein 2 Is Variably Expressed Across Excitatory and Inhibitory Neurons in Mouse Motor Circuits.

The Journal of comparative neurology·2026
Same journal

Brain Distribution of Orthopedia (Otp) Transcription Factor in Bony Fish: A Comparative Neuroanatomical Perspective.

The Journal of comparative neurology·2026
See all related articles

Related Experiment Video

Updated: May 21, 2026

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
10:09

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

Published on: June 7, 2019

Electron tomographic analysis of synaptic ultrastructure.

Alain C Burette1, Thomas Lesperance, John Crum

  • 1Department of Cell & Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA. alain.burette@gmail.com

The Journal of Comparative Neurology
|June 12, 2012
PubMed
Summary
This summary is machine-generated.

Electron tomography reveals the 3D structure of excitatory synapses in the rat brain. This advanced imaging technique provides new insights into the organization of synaptic nano-machines crucial for brain function.

More Related Videos

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex
12:06

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex

Published on: August 19, 2025

Related Experiment Videos

Last Updated: May 21, 2026

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
10:09

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

Published on: June 7, 2019

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex
12:06

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex

Published on: August 19, 2025

Area of Science:

  • Neuroscience
  • Cell Biology
  • Structural Biology

Background:

  • Synaptic function relies on protein interactions forming complex molecular machines.
  • Existing techniques offer limited insight into the precise 3D organization of these synaptic nano-machines.

Purpose of the Study:

  • To investigate the three-dimensional structural organization of axospinous synapses in the mammalian brain.
  • To utilize electron tomography for high-resolution imaging of synaptic architecture.

Main Methods:

  • Aldehyde fixation of adult rat neocortex and hippocampus.
  • Tannic acid stabilization as an alternative to osmium tetroxide postfixation.
  • High-resolution three-dimensional reconstruction using electron tomography.

Main Results:

  • Detailed structural visualization of axospinous synapses in the neocortex and hippocampus.
  • Identification of the supramolecular organization of synaptic protein complexes.
  • Novel insights into the spatial arrangement of components within excitatory synapses.

Conclusions:

  • Electron tomography offers unprecedented resolution for studying synaptic structure.
  • The findings provide a structural basis for understanding excitatory synaptic processing.
  • This study advances our knowledge of the nano-architecture underlying brain function.