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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
Published on: April 29, 2011
Trmt112 gene expression in mouse embryonic development.
Tiantian Gu1, Hongjuan He, Yan Zhang
1School of Life Science and Biotechnology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, No. 92 West Da-zhi Street, Harbin, 150001, Heilongjiang, China.
Acta Histochemica Et Cytochemica
|June 12, 2012
Summary
The mouse Trmt112 gene, crucial for RNA methylation, shows widespread expression during embryonic development. This suggests its involvement in the formation and function of various organs and tissues.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- The Trmt112 gene in mice is homologous to yeast TRM112, known for its role in RNA methylation.
- The precise function of mouse Trmt112 was initially unclear following its cloning.
Purpose of the Study:
- To investigate the expression patterns of the mouse Trmt112 gene during embryonic development.
- To understand the potential roles of Trmt112 in organogenesis and physiological functions.
Main Methods:
- Whole mount in situ hybridization was used to analyze Trmt112 expression in embryos.
- Quantitative real-time RT-PCR (QRT-PCR) was employed to quantify Trmt112 expression levels in various tissues.
Main Results:
- Trmt112 expression was high in the brain and nervous system from embryonic day 10.5 to 11.5.
- Abundant Trmt112 expression was detected in multiple organs, including the developing brain, thymus, lung, liver, intestine, kidney, and cartilage, at later developmental stages.
- QRT-PCR confirmed persistent Trmt112 expression throughout embryogenesis (E9.5-E18.5) and high levels in numerous organs at key developmental time points (E12.5, E15.5, E18.5).
Conclusions:
- The Trmt112 gene is ubiquitously and highly expressed during mouse embryonic development.
- The widespread expression pattern suggests Trmt112 plays a significant role in the morphogenesis of diverse organs and tissues.
- Trmt112 likely contributes to numerous essential physiological functions during embryonic development.
