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Related Concept Videos

PCR01:32

PCR

Overview
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

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Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
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Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc).

D Sugumar, L X Kong, Asma Ismail

    Biomicrofluidics
    |June 12, 2012
    PubMed
    Summary

    This study introduces a novel rotary-linear motion polymerase chain reaction (PCR) device for rapid, low-volume DNA amplification. The innovative compact disc design significantly reduces reaction times and reagent usage for multiple samples.

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    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
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    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

    Published on: November 14, 2018

    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Analytical Chemistry

    Background:

    • Conventional polymerase chain reaction (PCR) methods can be time-consuming and require significant reagent volumes.
    • Developing rapid and efficient DNA amplification techniques is crucial for various molecular biology applications.
    • Miniaturization and automation of PCR processes are key areas of research for improved throughput.

    Purpose of the Study:

    • To develop and evaluate a novel rotary-linear motion polymerase chain reaction (PCR) device for rapid, high-throughput DNA amplification.
    • To assess the efficiency, speed, and reagent consumption of the new PCR device compared to conventional methods.
    • To demonstrate the feasibility of using a compact disc platform for temperature-controlled, multi-sample PCR.

    Main Methods:

    • A novel rotary-linear motion PCR device was designed utilizing a compact disc platform with individually temperature-controlled sample chambers.
    • DNA amplification was achieved by shuttling samples between different temperature zones using the disc's combined rotary-linear movement.
    • A spring-loaded heater mechanism and a proportional-integral-derivative (PID) pulse width modulation control system were implemented for precise temperature management.

    Main Results:

    • The device successfully amplified up to 12 DNA samples simultaneously in under 30 minutes.
    • The reaction required a minimal sample volume of 5 μl.
    • Reagent volume was reduced by approximately 25% compared to conventional PCR methods.
    • Improved amplification rates and reduced overall reaction duration were observed.

    Conclusions:

    • The novel rotary-linear motion PCR device offers a rapid, efficient, and cost-effective solution for multi-sample DNA amplification.
    • The compact disc-based platform demonstrates significant potential for miniaturized and high-throughput molecular diagnostics.
    • This technology advancement contributes to reduced reagent consumption and faster turnaround times in molecular biology workflows.