[ELISA test-kit for herpes 2 serodiagnosis]

Area of Science:

• Immunology
• Virology
• Medical Diagnostics

Background:

• Herpes simplex virus 2 (HSV-2) infections pose diagnostic challenges.
• Differentiating primary infection from chronic disease or reactivation is crucial for effective management.
• Assessing antibody avidity can provide insights into the stage of infection.

Purpose of the Study:

• To develop and validate an Enzyme-Linked Immunosorbent Assay (ELISA) test kit for detecting HSV-2 specific IgG antibodies.
• To determine the avidity of these antibodies using the developed test kit.
• To evaluate the utility of the ELISA test kit in diagnosing different stages of HSV-2 infection.

Main Methods:

• Construction of an ELISA test kit utilizing recombinant HSV-2 glycoprotein gG2 (PSC SPC Diaproph-Med).
• Investigation of the distribution of specific IgG antibodies with varying avidity indexes in healthy donor samples.
• Application of the test kit for analyzing antibody profiles in different clinical scenarios.

Main Results:

• The developed ELISA test kit successfully detected Herpes simplex virus 2 specific IgG.
• The kit allowed for the determination of antibody avidity indexes.
• Analysis revealed the distribution of different avidity levels in the tested population.

Conclusions:

• The developed ELISA test kit is a viable tool for detecting HSV-2 specific IgG and assessing antibody avidity.
• This test kit can aid in confirming primary HSV-2 infections.
• The kit shows potential for differentiating between primary infection, chronic disease, and viral reactivation, serving as an additional diagnostic aid for herpetic infections.