Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Technical variability of bone histomorphometric measurements.

E Bonucci1, P Ballanti, C Della Rocca

  • 1Department of Human Biopathology, La Sapienza University of Rome, Italy.

Bone and Mineral
|November 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Relationship between formaldehyde exposure, respiratory irritant effects and cancers: a review of reviews.

Public health·2023
Same author

Nutrition education in medical schools (NEMS) project: Joining ESPEN and university point of view.

Clinical nutrition (Edinburgh, Scotland)·2021
Same author

Mesenchymal cystic hamartoma presenting with pneumothorax: case report and review of the literature.

General thoracic and cardiovascular surgery·2020
Same author

The 16, 18, and 45 HPV infection in high grade squamous cervical lesions in primary hr-HPV test screening program.

European journal of gynaecological oncology·2016
Same author

Primary HPV test screening in cervical cancer: a two-year experience of a single screening center in Latina (Italy).

European journal of gynaecological oncology·2015
Same author

Ezrin-related Phosphoinositide pathway modifies RhoA and Rac1 in human osteosarcoma cell lines.

Journal of cell communication and signaling·2015

Microscopic field variation significantly impacts bone biopsy analysis accuracy. Increasing microscopic fields analyzed improves precision for bone volume and thickness measurements, crucial for accurate bone health assessment.

Area of Science:

  • Bone Histomorphometry
  • Skeletal Biology
  • Biomedical Engineering

Background:

  • Accurate bone biopsy analysis is critical for diagnosing and monitoring bone diseases.
  • Understanding sources of variability in bone histomorphometry is essential for reliable results.

Purpose of the Study:

  • To quantify the main sources of variability in bone biopsy sample analysis.
  • To identify the contribution of different factors to measurement error in bone histomorphometry.

Main Methods:

  • Analysis of double iliac crest biopsies from 12 subjects.
  • Estimation of variance components using ANOVA for random effects.
  • Measurement of bone histomorphometric parameters including bone volume (BV/TV) and osteoid thickness (O.Th).

Related Experiment Videos

Main Results:

  • Variation between microscopic fields was the largest contributor to measurement error for most parameters (e.g., >80% for OV/BV).
  • Distance from compact bone influenced BV/TV (40%) and O.Th (10%) variability.
  • Analyzing 12 fields for BV/TV and 48 fields for other parameters significantly reduced variability.

Conclusions:

  • Microscopic field variation is the primary source of error in bone histomorphometry.
  • Optimizing the number of microscopic fields analyzed enhances measurement precision.
  • Standardizing the number of fields analyzed is recommended for consistent bone biopsy interpretation.