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Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
Published on: July 22, 2021
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Eckol inhibits ultraviolet B-induced cell damage in human keratinocytes via a decrease in oxidative stress.
Mei Jing Piao1, Nam Ho Lee, Sungwook Chae
1School of Medicine and Veterinary Medical Research Institute, Jeju National University, Republic of Korea.
Biological & Pharmaceutical Bulletin
|June 13, 2012
Summary
Eckol demonstrates significant antioxidant effects, protecting human skin cells from UVB damage by reducing reactive oxygen species (ROS) and preventing apoptosis. This natural compound offers a promising approach for mitigating UV-induced skin damage.
Area of Science:
- Dermatology
- Biochemistry
- Photobiology
Background:
- Eckol, a phlorotannin from Ecklonia cava, is known for its antioxidant properties.
- Previous studies indicated eckol protects against oxidative stress induced by hydrogen peroxide and gamma rays.
- UVB radiation is a significant cause of oxidative stress and cellular damage in human skin.
Purpose of the Study:
- To investigate the protective role of eckol against UVB-induced oxidative damage in human skin keratinocytes (HaCaT cells).
- To compare the antioxidant activity of eckol with triphlorethol-A and quercetin based on chemical structure.
- To elucidate the mechanisms by which eckol exerts its protective effects against UVB radiation.
Main Methods:
- Human skin keratinocytes (HaCaT) were exposed to UVB radiation.
- Eckol, triphlorethol-A, and quercetin were used to assess antioxidant activity.
- Measurements included intracellular reactive oxygen species (ROS) levels, cellular component injury, cell viability, and apoptosis.
- Mitochondrial membrane potential disruption was assessed to evaluate apoptosis inhibition.
Main Results:
- Eckol significantly decreased UVB-induced intracellular ROS production in HaCaT cells.
- Eckol reduced cellular component injury and restored cell viability following UVB exposure.
- Eckol inhibited UVB-induced apoptosis by preventing the disruption of mitochondrial membranes.
- Eckol exhibited potent antioxidant activity, comparable to or exceeding that of quercetin in this context.
Conclusions:
- Eckol effectively protects human keratinocytes against UVB-induced oxidative stress.
- The protective mechanism involves scavenging ROS and preserving cellular integrity.
- Eckol demonstrates potential as a photoprotective agent for skin health.
