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Updated: May 21, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
In vivo functional analysis of transcription factor: response element interaction using transgenic Xenopus laevis
Heithem M El-Hodiri1, Yi Pan, Lisa E Kelly
1Center for Molecular and Human Genetics, The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA. Heithem.El-Hodiri@NationwideChildrens.org
Abstract:
Analysis of transcription factor-target interactions in vivo is important to the study of transcriptional regulation of gene expression. A key experiment involves analysis of the functional interaction between a trans-acting factor and its corresponding cis-acting element in the context of a target promoter in vivo. We describe a method for this analysis in transgenic Xenopus tadpoles in which expression of the trans-acting factor is knocked down using an shRNA-mediated approach.
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