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From cloning to a commercial realization: human alpha interferon.

E Baron¹, S Narula

¹Georgetown University School of Medicine, Washington, D.C.

Critical Reviews in Biotechnology

|January 1, 1990

Summary

Recombinant DNA technology enabled the production of Interferon Alfa-2B (IFN alpha-2b), a key therapeutic. This review covers its discovery, cloning, and expression as an early biopharmaceutical.

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Area of Science:

Biotechnology
Molecular Biology
Pharmacology

Background:

Recombinant DNA technology allows for the production of valuable biologicals.
Interferon Alfa-2B (IFN alpha-2b) is a significant therapeutic agent.

Purpose of the Study:

To review the discovery, characterization, cloning, and expression of Interferon Alfa-2B.
To provide insights into one of the first recombinant DNA-based drugs.

Main Methods:

Review of scientific literature on Interferon Alfa-2B.
Analysis of studies on gene expression and purification.
Examination of biological and clinical data.

Main Results:

Successful cloning and expression of Interferon Alfa-2B (IFN alpha-2b) were achieved.
IFN alpha-2b became a commercially available therapeutic (Intron A).
IFN alpha-2a (Roferon) is another related interferon product.

Conclusions:

Interferon Alfa-2B represents a landmark achievement in recombinant DNA therapeutics.
The comprehensive studies offer valuable information on early biopharmaceutical development.
This review highlights the impact of genetic engineering on medicine.

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