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Updated: May 21, 2026

A Rhodopsin Transport Assay by High-Content Imaging Analysis
Published on: January 16, 2019
In vitro biochemical assays to monitor rhodopsin function
Joshua Sammons1, Alecia K Gross
1Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
Abstract:
Rhodopsin is the dim-light photoreceptor responsible for initiation of the visual transduction cascade. In the dark its activity is very low, while light activation catalyzes the activation of its G-protein transducin. The first step in resetting rhodopsin and the phototransduction cascade involves the phosphorylation of light-active rhodopsin by rhodopsin kinase. Here, we describe assays to monitor the function of rhodopsin or rhodopsin mutants.
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