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Stimulation of actin synthesis in phalloidin-treated cells. Evidence for autoregulatory control
A S Serpinskaya1, O N Denisenko, V I Gelfand
1Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.
Abstract:
We used the technique of scrape loading to introduce phalloidin into mouse embryo fibroblasts in mass culture. Phalloidin almost completely destroyed actin microfilament bundles, but the amount of polymerized cytoskeleton-associated actin was increased approximately two-fold and the amount of monomeric (Triton X-100 extractable) actin was significantly reduced. The major result of the present study is that the rate of actin synthesis in the phalloidin-treated cells was 2-3 times higher than in the control cells. Northern blot and translation in a cell-free system from rabbit reticulocytes showed that the actin mRNA level significantly increased as a result of phalloidin treatment.