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Updated: May 21, 2026

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Published on: September 20, 2016
[Influence of stressors on formation of uncultured microorganism forms and their recovery]
Abstract:
Data of the last decade on the studies of influence of various inductors of microorganism uncultured state and reasons of reversion that include not only effect of appropriate cultivation conditions but also simple cancellation of unfavorable effects are analyzed. Reasons for transition to uncultured state are discussed; those could be a variety of factors such as heat, alkaline, acid and osmotic stress. Factors that promote recovery from uncultured state with the main being Rpf (Resustication promoting factor) factor isolated from Micrococcus luteus and cancellation of endogenic peroxide effect by addition of catalase and sodium pyruvate are analyzed.
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