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Updated: May 21, 2026
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Isolation, identification and bioactivity of endophytic fungi from medicinal plant Malus sieboldii
1Ethnic Pharmaceutical Institute, Southwest University for Nationalities, Chengdu 610041, China.
Objective:
To isolate and identify endophytic fungi from Malus sieboldii, and detect cytotoxicity, protease inhibition and antifungal activities of their crude extracts.
Method:
The fungi were identified with the aid of morphology or Internal Transcribed Spacer (ITS) rDNA molecular methods. Fungal activities were tested by cylinder-plate, MTT and BRpNA methods, respectively.
Result:
A total of 217 endophytic fungi were isolated from M. sieboldii. Of the 22 taxa obtained, non-sporulating, Alternaria, Colletotrichum, Aspergillu, Fusarlum, Gliocladium and Cunninghamella were dominant communities. The result of the bioactivity test showed that 30 endophytic fungi displayed inhibition against at least one pathogenic fungus, and 3 and 4 showed cytotoxicity and protease inhibition, respectively.
Conclusion:
M. sieboldii should be a potential source of bioactive endophytic fungi.
