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Updated: May 21, 2026

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
Published on: October 9, 2016
A novel splice variant of folate receptor 4 predominantly expressed in regulatory T cells
Yi Tian1, Guoqiang Wu, Jun-Chao Xing
1Institute of Immunology, PLA, Third Military Medical University, Chongqing, Peoples Republic China.
Background:
Regulatory T cells (Tregs) are required for proper maintenance of immunological self-tolerance and immune homeostasis. Folate receptor 4 (FR4) is expressed at high levels in transforming growth factor-beta (TGF-β)-induced Tregs and natural Tregs. Moreover, antibody-mediated targeting of FR4 is sufficient to mediate Treg depletion.
Results:
In this study, we describe a novel FR4 transcript variant, FR4D3, in which exon 3 is deleted. The mRNA of FR4D3 encodes a FR4 variant truncated by 189 bp. FR4D3 was found to be predominantly expressed in CD4(+)CD25(+) Treg cells. Overexpression of FR4D3 in CD4(+)CD25(+) Treg cells in vitro stimulated proliferation, which may modulate the ability of these cells to bind and incorporate folic acid.
Conclusions:
Our results suggested that high levels of FR4D3 may be critical to support the substantial proliferative capacity of Treg cells.
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