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Related Concept Videos

Rab Cascades01:25

Rab Cascades

3.4K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

6.3K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.3K
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

5.8K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
5.8K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

3.3K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.3K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.4K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Related Experiment Video

Updated: Jan 19, 2026

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
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Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

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A novel function of Rab5 in mitosis.

Letizia Lanzetti1

  • 1Department of Oncological Sciences, University of Turin, Candiolo, Turin, Italy. letizia.lanzetti@ircc.it

Small Gtpases
|June 15, 2012
PubMed
Summary

Mitotic spindle assembly and stability rely on membrane trafficking proteins. However, their precise roles and requirements during cell division remain largely unclear.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Membrane trafficking proteins are implicated in various cellular processes.
  • Their involvement in spindle assembly and stability during mitosis is an emerging area of research.
  • The precise functions of many of these proteins in mitosis are not well-defined.

Purpose of the Study:

  • To investigate the requirement of specific membrane trafficking proteins in mitosis.
  • To elucidate the roles of these proteins in spindle assembly and stability.
  • To enhance the understanding of molecular mechanisms governing cell division.

Main Methods:

  • Utilized cell-based assays to study protein localization and function.
  • Employed microscopy techniques to visualize spindle dynamics.

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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  • Investigated protein interactions and cellular localization during mitosis.

    • Main Results:

    • Identified key membrane trafficking proteins essential for mitotic spindle function.
    • Demonstrated the involvement of these proteins in maintaining spindle integrity.
    • Observed specific defects in spindle assembly and stability upon perturbation of these proteins.

    Conclusions:

    • Membrane trafficking proteins play critical roles in ensuring accurate chromosome segregation.
    • Understanding these proteins advances knowledge of cell cycle regulation.
    • Further research into these proteins could reveal new therapeutic targets for diseases involving cell division.