Selective light-triggered chemiluminescence between fluorescent dyes and luminol, and its analytical application

Area of Science:

• Analytical Chemistry
• Photochemistry
• Biomedical Science

Background:

• Chemiluminescence (CL) is a widely used analytical technique.
• Current methods for triggering CL reactions often involve physical interference, limiting selectivity and resolution.
• There is a need for novel CL triggering methods with enhanced spatial and temporal control.

Purpose of the Study:

• To report a novel photoinduced chemiluminescence (PICL) phenomenon.
• To propose a mechanism for the PICL reaction.
• To highlight the advantages of the in-situ PICL method for analytical applications.

Main Methods:

• Irradiation of luminol solutions containing fluorescent dyes (hematoporphyrin, fluorescein, eosin, methylene blue) with light.
• Investigation of the photoinduced chemiluminescence (PICL) reaction mechanism.
• Application of the PICL method for the analysis of adrenaline in urine samples.

Main Results:

• Demonstrated a novel chemiluminescence phenomenon triggered by light irradiation in the presence of fluorescent dyes.
• Proposed a mechanism for the photoinduced chemiluminescence (PICL) reaction.
• Achieved selective and high-resolution detection of adrenaline in urine with a linear range of 2.0 × 10⁻¹⁰–1.0 × 10⁻⁷ g mL⁻¹ and a detection limit of 6.0 × 10⁻¹¹ g mL⁻¹.

Conclusions:

• The in-situ PICL method offers significant advantages, including enhanced selectivity, spatial, and temporal resolution, and no physical interference.
• The PICL method has broad potential applications in analytical sciences.
• The developed method enables sensitive and selective detection of adrenaline.