Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Total Internal Reflection Fluorescence Microscopy

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Planning Non-Linear Trajectories for No-Touch Thermal Ablation Using Passive Steerable Needle With Controlled Stiffness and PSAM.

Healthcare technology letters·2025

Same author

Safety and efficacy of apixaban versus warfarin in peritoneal dialysis patients with non-valvular atrial fibrillation: protocol for a prospective, randomised, open-label, blinded endpoint trial (APIDP2).

BMJ open·2024

Same author

Diatom DNA metabarcoding for ecological assessment: Comparison among bioinformatics pipelines used in six European countries reveals the need for standardization.

The Science of the total environment·2020

Same author

Chloroplast DNA variation in a hyperdiverse tropical tree community.

Ecology and evolution·2019

Same author

Separation efficiency of a hydrodynamic separator using a 3D computational fluid dynamics multiscale approach.

Water science and technology : a journal of the International Association on Water Pollution Research·2014

Same author

Optimization of a hydrodynamic separator using a multiscale computational fluid dynamics approach.

Water science and technology : a journal of the International Association on Water Pollution Research·2013

Search research articles

Related Experiment Video

Updated: May 21, 2026

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Aperture effect correction in spectroscopic full-field optical coherence tomography.

Antoine Morin¹, Jean-Marc Frigerio

¹Université Pierre et Marie Curie, UMR 7588, INSP, 4 Place Jussieu Paris F-75005, France. antoine.morin@insp.jussieu.fr

|June 15, 2012

Summary

Full-field spectroscopic optical coherence tomography (OCT) reveals field-dependent spectral shifts due to varying numerical aperture. A novel correction method addresses these shifts for accurate white-light spectroscopy in turbid media.

More Related Videos

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Related Experiment Videos

Last Updated: May 21, 2026

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Area of Science:

Optical Physics
Spectroscopy
Biomedical Optics

Background:

Spatially resolved spectroscopic optical coherence tomography (OCT) is valuable for spectral analysis in turbid media.
Full-field OCT configurations can exhibit field-dependent spectral shifts.

Purpose of the Study:

To investigate field-dependent spectral shifts in full-field OCT using a Mirau objective.
To develop and validate a numerical correction method for white-light spectroscopy in OCT.

Main Methods:

Theoretical fitting of interferograms using a general Mirau interference formula.
Implementation of a numerical correction method for white-light spectroscopy.
Testing the method on a plane gold sample for reflectivity measurement.

Main Results:

Identified field-dependent spectral shifts in full-field OCT due to effective numerical aperture variation.
Successfully demonstrated the proposed numerical correction method.
Validated the method by accurately measuring the reflectivity of a gold sample.

Conclusions:

The developed numerical correction method effectively compensates for field-dependent spectral shifts in full-field OCT.
This advancement enables more accurate spectral analysis in turbid media using white-light OCT.