Imaging of oocyte development using ultrahigh-resolution full-field optical coherence tomography

Area of Science:

• Biomedical Optics
• Developmental Biology
• Imaging Science

Background:

• Observing early embryonic development requires high-resolution imaging techniques.
• Existing methods may lack the necessary resolution or in vivo capabilities for detailed developmental studies.

Purpose of the Study:

• To develop and demonstrate an ultrahigh-resolution white-light full-field optical coherence tomography system for in vivo mouse embryo imaging.
• To achieve high spatial resolution and detection sensitivity for observing dynamic developmental processes.

Main Methods:

• Development of a white-light full-field optical coherence tomography system.
• Utilizing an extremely short coherence length light source and optimized dispersion mismatch compensation.
• Implementing a homemade incubator for maintaining physiological conditions (CO2, temperature, humidity) during in vivo imaging.
• Employing an electronic light shutter to minimize light exposure to the embryo.

Main Results:

• Achieved spatial resolution of 1.8μm (transverse) × 1.12μm (axial).
• Obtained a shot-noise limited detection sensitivity of 80 dB.
• Demonstrated in vivo time-series 2D imaging of mouse oocytes during in vitro fertilization (germinal vesicles stage).

Conclusions:

• The developed white-light full-field optical coherence tomography system provides unprecedented resolution for mouse embryo development studies.
• The system's in vivo capabilities and high sensitivity are crucial for monitoring dynamic embryonic processes.
• This technology offers a powerful tool for advancing research in reproductive biology and developmental science.