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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

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Advanced FRET methodologies: protein-lipid selectivity detection and quantification

Fábio Fernandes1, Manuel Prieto, Luís M S Loura

  • 1Institute of Nanosciences and Nanotechnologies, Lisbon, Portugal. fernandesf@ist.utl.pt

Advances in Experimental Medicine and Biology
|June 15, 2012
PubMed
Summary

No abstract available in PubMed .

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