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Propagating and Detecting an Infectious Molecular Clone of Maedi-visna Virus that Expresses Green Fluorescent Protein
Published on: October 9, 2011
Ovine TRIM5α can restrict visna/maedi virus
P Jáuregui1, H Crespo, I Glaria
1Instituto de Agrobiotecnología, CSIC-UPNA-Gobierno de Navarra, Mutilva Baja, Navarra, Spain.
None:
The restrictive properties of tripartite motif-containing 5 alpha (TRIM5α) from small ruminant species have not been explored. Here, we identify highly similar TRIM5α sequences in sheep and goats. Cells transduced with ovine TRIM5α effectively restricted the lentivirus visna/maedi virus DNA synthesis. Proteasome inhibition in cells transduced with ovine TRIM5α restored restricted viral DNA synthesis, suggesting a conserved mechanism of restriction. Identification of TRIM5α active molecular species may open new prophylactic strategies against lentiviral infections.
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