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Silk fibroin films show structural changes based on thickness and substrate contact. Thicker films exhibit layered structures, while thinner films display unique swelling behavior due to nanobubble trapping.

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Area of Science:

  • Materials Science
  • Biomaterials Engineering
  • Surface Science

Background:

  • Bombyx mori silk fibroin is a promising biomaterial for thin films.
  • Understanding the structural organization of silk fibroin films is crucial for their application.
  • Ultrathin films present unique properties compared to bulk materials.

Purpose of the Study:

  • To investigate the internal structural organization of Bombyx mori silk fibroin films.
  • To analyze the structural differences in dry and swollen states.
  • To understand the influence of substrate interaction and film thickness on silk fibroin structure.

Main Methods:

  • Neutron reflectivity (NR) measurements were employed to probe film structure.
  • Atomic force microscopy (AFM) provided surface morphology information.
  • Ellipsometry was used to determine film thickness and optical properties.
  • Spin-assisted layer-by-layer (SA-LbL) technique was used for film deposition.

Main Results:

  • Monolayer silk films exhibited random nanofibril organization.
  • Thicker multilayer films showed vertical segregation with distinct structural regions.
  • Interfacial layers near the silicon oxide surface displayed beta-sheet crystallization and nanoporous morphology.
  • Thicker films delaminated in D(2)O during swelling.
  • A 6 nm silk monolayer showed a 60% thickness increase and density decrease in a liquid environment, attributed to air nanobubble trapping.

Conclusions:

  • Silk fibroin films possess a complex internal structure influenced by deposition method and film thickness.
  • The secondary structure of silk fibroin varies within multilayer films, with interfacial crystallization occurring.
  • The nanoporous nature of silk films leads to unique swelling behavior and potential artifacts like nanobubble trapping.