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Related Experiment Video

Updated: May 21, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

B-cell depletion therapy in patients with diffuse systemic sclerosis associates with a significant decrease in PDGFR

Dimitrios Daoussis¹, Athanassios C Tsamandas, Stamatis-Nick C Liossis

¹Division of Rheumatology, Department of Internal Medicine, Patras University Hospital, University of Patras Medical School, Patras, Rion, 26504, Greece. jimdaoussis@hotmail.com

Arthritis Research & Therapy

|June 16, 2012

Summary

Rituximab (RTX) treatment may reduce skin fibrosis in systemic sclerosis (SSc) by decreasing platelet-derived growth factor receptor (PDGFR) activation. This suggests RTX has a disease-modifying role in SSc.

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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

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Published on: April 8, 2016

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

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Published on: November 4, 2016

Area of Science:

Immunology
Dermatology
Rheumatology

Background:

Systemic sclerosis (SSc) is a fibrotic disease.
Rituximab (RTX) shows promise in treating SSc.
The mechanism of RTX's effect on fibrosis is being investigated.

Purpose of the Study:

To explore if RTX reduces fibrosis in SSc by affecting the platelet-derived growth factor receptor (PDGFR) pathway.
To assess changes in PDGFR expression and activation after RTX treatment in SSc patients.

Main Methods:

Skin biopsies from SSc patients before and after RTX treatment were analyzed.
Immunohistochemistry was used to measure PDGFR, phosphorylated PDGFR, and platelet-derived growth factor.
Expression levels were compared between treated patients, control SSc patients, and healthy subjects.

Main Results:

PDGFRα and PDGFRβ expression strongly correlated with fibrosis in SSc skin.
RTX treatment significantly decreased PDGFRα and PDGFRβ expression in the papillary dermis.
Phosphorylated PDGFRα and PDGFRβ levels also significantly decreased after RTX administration.

Conclusions:

RTX may improve skin fibrosis in SSc by reducing PDGFR pathway activation.
These findings support a disease-modifying potential for RTX in SSc.
Larger studies are needed to confirm RTX efficacy in SSc.