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Related Experiment Videos

Nicking-closing activity associated with bacteriophage lambda int gene product.

Y Kikuchi, H A Nash

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1979
    PubMed
    Summary

    Bacteriophage lambda recombination relies on the Int protein, which possesses a DNA nicking-closing activity. This activity is linked to recombination and may facilitate strand exchange during the process.

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    Area of Science:

    • Molecular Biology
    • Virology
    • Biochemistry

    Background:

    • Integrative recombination in bacteriophage lambda is a critical process for viral integration.
    • This recombination is mediated by the Int protein, encoded by the phage int gene.

    Purpose of the Study:

    • To investigate the biochemical activities of the highly purified Int protein.
    • To establish the association between the nicking-closing activity and the recombination function of Int.
    • To propose a model for Int's role in recombination.

    Main Methods:

    • Purification of bacteriophage lambda Int protein.
    • Assays for DNA nicking-closing activity and integrative recombination.
    • Analysis of Int activity under various conditions (heat, antiserum, mutant extracts, cation presence/absence).

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    Main Results:

    • Highly purified Int protein exhibits DNA nicking-closing activity.
    • This activity co-sediments with recombination activity and is inactivated in parallel by heat and antiserum.
    • The nicking-closing activity functions without divalent cations or apparent energy source and is inhibited by Mg2+, spermidine, and single-stranded DNA.
    • Int relaxes both positive and negative supercoils.

    Conclusions:

    • The nicking-closing activity is intrinsically associated with the Int protein.
    • This DNA-modifying activity is likely essential for the strand exchange mechanism during bacteriophage lambda integrative recombination.
    • A model is proposed to explain how Int's nicking-closing function facilitates recombination.