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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: May 21, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Multicolor super-resolution DNA imaging for genetic analysis.

Murat Baday¹, Aaron Cravens, Alex Hastie

¹Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.

|June 16, 2012

Summary

This summary is machine-generated.

We developed a low-cost, high-resolution DNA imaging technique to map gene variations linked to diseases. This method achieves 100 bp resolution, aiding in genetic variance analysis and disease diagnosis.

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3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

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Last Updated: May 21, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Published on: January 25, 2020

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Genetic variations and abnormalities are primary causes of various cancers and neurodegenerative diseases.
Accurate detection of structural variations in the genome is crucial for understanding and diagnosing genetic disorders.

Purpose of the Study:

To develop a high-resolution, high-throughput, and cost-effective imaging technique for mapping structural gene variations.
To demonstrate the capability of the technique in resolving densely packed DNA nicking sites.

Main Methods:

Mapping of Nb.BbvCI endonuclease enzyme nicking sites on lambda DNA.
Digestion of human BAC DNA using Nb.BsmI and Nb.BbvCI endonuclease enzymes.
High-resolution imaging to resolve densely labeled nicking sites, even those closer than the diffraction limit.

Main Results:

Successfully mapped all seven nicking sites of Nb.BbvCI on lambda DNA.
Resolved densely labeled patterns of 107 nicking sites on human BAC DNA.
Achieved detailed DNA nicking site mapping with a resolution of 100 base pairs (bp).

Conclusions:

The developed imaging technique provides high-resolution DNA mapping.
This technique has the potential to reveal critical information about genetic variance.
Facilitates improved medical diagnosis for a range of genetic diseases.