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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 21, 2026

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

Genomic chondrocyte culture profiling by array-CGH, interphase-FISH and RT-PCR.

M Stumm¹, E Boger, C G Gaissmaier

¹BG Berlin-Genetics GmbH, MDC-Buch, Berlin, Germany. stumm@kudamm-199.de

Osteoarthritis and Cartilage

|June 16, 2012

Summary

This summary is machine-generated.

Cultured chondrocytes from male donors may lose their Y-chromosome with age, but this does not affect cell function. Caution is advised when using chondrocytes from older male patients for tissue engineering.

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Last Updated: May 21, 2026

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

Area of Science:

Cell Biology
Genomics
Tissue Engineering

Background:

In vitro expansion of chondrocytes is crucial for cartilage tissue engineering.
Prolonged cell culture may induce genomic alterations.
Understanding these changes is vital for clinical applications.

Purpose of the Study:

To investigate in vitro induced genomic changes in human chondrocytes.
To assess the impact of cell expansion on chondrocyte genomes.
To evaluate potential functional consequences of genomic alterations.

Main Methods:

Array comparative genomic hybridization (aCGH) and fluorescence in situ hybridization (FISH) were used for molecular karyotyping.
Analysis of DNA from 25 chondrocyte samples (9 donors) and 28 samples (16 individuals).
Reverse-transciptase polymerase chain reaction (RT-PCR) assessed chondrocytic mRNA phenotype.

Main Results:

Autosomal stability was observed in all samples.
Male samples showed variable loss of the Y-chromosome, potentially age-dependent.
No correlation between Y-chromosome loss and mRNA expression of collagen types I, II, aggrecan, or IL-1ß was found.

Conclusions:

Cultured chondrocytes from male donors may experience age-related Y-chromosome loss.
This loss did not appear to cause functional impairment in the studied chondrocytes.
Caution is recommended when using chondrocytes from elderly male patients in tissue engineering.