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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

[BEX2 regulates cell cycle through the interaction with INI1/hSNF5].

Qiu-Yue Han1, Yan-Hui Fan, Ya-Li Wang

  • 1The Ninth Hospital of Shijiazhuang, Shijiazhuang 050100, China.

Yi Chuan = Hereditas
|June 16, 2012
PubMed
Summary

Brain expressed X-linked protein 2 (BEX2) interacts with INI1/hSNF5, a component of the SWI/SNF complex. This interaction, occurring in the nucleus, influences cell cycle regulation and gene expression.

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Published on: September 26, 2025

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Brain expressed X-linked protein 2 (BEX2) is highly expressed in the brain and testis, with expression levels changing during embryonic development.
  • The precise function of BEX2 remains largely uncharacterized.
  • INI1/hSNF5 is a crucial component of the SWI/SNF chromatin remodeling complex.

Purpose of the Study:

  • To identify binding partners of BEX2.
  • To elucidate the functional significance of the BEX2-INI1/hSNF5 interaction.

Main Methods:

  • Yeast two-hybrid screening to identify BEX2 interacting proteins.
  • GST Pull-down assays to confirm direct and specific protein-protein interactions.
  • Truncated mutation analysis to map interaction domains.
  • Sub-cellular localization studies.
  • Cell cycle analysis via co-overexpression.

Main Results:

  • INI1/hSNF5 was identified as a binding partner of BEX2.
  • The interaction between BEX2 and INI1/hSNF5 is direct, specific, and physical.
  • Two conserved reverse repeat sequences in INI1/hSNF5 are essential for binding BEX2.
  • Both proteins predominantly localize to the cell nucleus.
  • Co-overexpression of BEX2 and INI1/hSNF5 leads to an increase in cells within the S phase of the cell cycle.

Conclusions:

  • BEX2 interacts with INI1/hSNF5, suggesting a role in the SWI/SNF complex.
  • The BEX2-INI1/hSNF5 interaction occurs in the nucleus and may be involved in gene expression regulation.
  • BEX2 influences cell cycle progression, potentially through its interaction with INI1/hSNF5.