Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Unified Mass Spectrometry Workflow Integrating Bottom-Up and Native Top-Down Proteomics via Nanodroplet Enzymatic Digestion.

Analytical chemistry·2026
Same author

Enhanced label-free SERS detection of uremic toxins in clinical spent dialysate.

Analytica chimica acta·2026
Same author

Cervical spondylosis with propriospinal myoclonus and vocalization: a case report.

Frontiers in surgery·2026
Same author

IgG Gal-ratio, a promising serum biomarker for detecting ovarian cancer in as early as stage I disease.

Journal of ovarian research·2026
Same author

Harnessing solid CO<sub>2</sub> in hybrid alkaline cement: Dry ice as a pathway to high-performance and low-emission materials.

Environmental research·2026
Same author

Theoretical Study of High-Order Velocity Focusing Achieved with Single-Stage Reflectron Time-of-Flight Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2025
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: May 21, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Macromolecular ion accelerator.

Yun-Fei Hsu1, Jung-Lee Lin, Szu-Hsueh Lai

  • 1Genomics Research Center, Academia Sinica, Nankang, Taipei, Taiwan.

Analytical Chemistry
|June 16, 2012
PubMed
Summary
This summary is machine-generated.

A new macromolecular ion accelerator (MIA) was developed for stepwise ion acceleration. This instrument successfully detected ions with a mass-to-charge ratio up to 30,000,000, demonstrating higher kinetic energy for macromolecules.

More Related Videos

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Preparing an Isotopically Pure 229Th Ion Beam for Studies of 229mTh
10:42

Preparing an Isotopically Pure 229Th Ion Beam for Studies of 229mTh

Published on: May 3, 2019

Related Experiment Videos

Last Updated: May 21, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

Preparing an Isotopically Pure 229Th Ion Beam for Studies of 229mTh
10:42

Preparing an Isotopically Pure 229Th Ion Beam for Studies of 229mTh

Published on: May 3, 2019

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Instrumentation Science

Background:

  • Macromolecular ions are crucial in various scientific fields.
  • Existing acceleration techniques have limitations for high mass-to-charge ratio ions.

Purpose of the Study:

  • To develop a novel instrument for accelerating macromolecular ions.
  • To demonstrate the capability of detecting high mass-to-charge ratio ions.

Main Methods:

  • Development of a macromolecular ion accelerator (MIA) using consecutive planar electrodes.
  • Utilizing Matrix-Assisted Laser Desorption/Ionization (MALDI) for ion generation.
  • Employing a Z-gap microchannel plate (MCP) detector for signal recording.

Main Results:

  • Successful detection of ions with a mass-to-charge (m/z) ratio up to 30,000,000.
  • Demonstration of accelerating singly charged biomolecular ions with voltages approaching 1 MV.
  • Evidence of significantly higher kinetic energy achievable for macromolecular ions.

Conclusions:

  • The developed macromolecular ion accelerator (MIA) is effective for high m/z ions.
  • This advancement enables the study of macromolecular ions with unprecedented kinetic energies.
  • The MIA technology opens new avenues in mass spectrometry and related research.