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Related Experiment Video

Updated: May 21, 2026

In Vitro Analysis of PDZ-dependent CFTR Macromolecular Signaling Complexes

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Published on: August 13, 2012

The cytoplasmic AID complex.

Julien Häsler¹, Cristina Rada, Michael S Neuberger

¹Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK. julien.hasler@gmail.com

Seminars in Immunology

|June 16, 2012

Summary

This summary is machine-generated.

Most Activation Induced Deaminase (AID) protein resides in the cytoplasm, forming a complex with translation elongation factor 1α (eEF1A). This review explores the cytoplasmic AID complex and its potential roles in regulating nuclear import.

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Area of Science:

Immunology
Molecular Biology
Cell Biology

Background:

Activation Induced Deaminase (AID) is crucial for antibody gene diversification.
Despite its nuclear function, significant AID protein is localized in the cytoplasm.
The cytoplasmic localization of AID suggests regulatory mechanisms controlling its nuclear access.

Purpose of the Study:

To review current knowledge on the cytoplasmic AID complex.
To discuss the composition and characteristics of the cytoplasmic AID complex.
To explore potential functions of the cytoplasmic AID complex in regulating AID nuclear import.

Main Methods:

Literature review of studies on AID localization and function.
Analysis of data regarding the size and composition of the cytoplasmic AID complex.
Speculative analysis of protein-protein interactions involving AID and chaperones.

Main Results:

The cytoplasmic AID complex is estimated to be 300-500 kDa (10-11S).
Translation elongation factor 1α (eEF1A) is a major component of the cytoplasmic AID complex.
Interactions with chaperones are implicated in AID regulation.

Conclusions:

The cytoplasmic AID complex, including eEF1A, likely plays a role in regulating AID's localization.
This complex may control the retention of AID in the cytoplasm and its subsequent release for nuclear import.
Understanding the cytoplasmic AID complex is key to elucidating the regulation of antibody gene diversification.