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Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: May 21, 2026

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
09:25

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Published on: October 14, 2016

[A simple method for isolating and culturing single cancer stem cells].

Yongjian Deng1, Qiang Jiang, Lina Yu

  • 1Department of Pathology, Southern Medical University, Guangzhou, China. dengyj@fimmu.com

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|June 16, 2012
PubMed
Summary

This study presents a novel method for isolating single cancer stem cells (CSCs) using a custom apparatus. Microdrop culture proved effective and stable for culturing these CSCs, offering a promising approach for cancer research.

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Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
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Last Updated: May 21, 2026

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
09:25

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

Published on: October 14, 2016

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
07:01

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Published on: March 1, 2020

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
09:06

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

Published on: December 13, 2019

Area of Science:

  • Cell Biology
  • Cancer Research
  • Stem Cell Biology

Context:

  • Cancer stem cells (CSCs) are crucial for tumor initiation and progression.
  • Effective isolation and culture of single CSCs are vital for studying their properties and developing therapies.
  • Current methods for CSC isolation and culture can be challenging and inefficient.

Purpose:

  • To develop and validate an efficient method for isolating and culturing single cancer stem cells (CSCs) from the SW480 cell line.
  • To assess the efficacy of a newly developed capillary glass tube-based separation apparatus for CSC isolation.
  • To compare the performance of microdrop culture versus 96-well plate culture for maintaining CSC viability and proliferation.

Summary:

  • A novel apparatus using capillary glass tubes was developed for isolating single cancer stem cells (CSCs).
  • The SW480 cell line was used, with CSCs identified by CD133 and CK7 markers.
  • The isolation success rate was 98.99%, and microdrop culture demonstrated convenience and stability for CSC cultivation, comparable to 96-well plates in early divisions.

Impact:

  • This method facilitates the study of individual cancer stem cells, enabling deeper understanding of tumor heterogeneity.
  • The developed technique provides a reliable and efficient tool for cancer stem cell research.
  • Microdrop culture is recommended as a primary method for single cancer stem cell culture due to its convenience and stability.