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An extended QBASIC program for the normalization and computation of whole-cell protein profiles and the application
E J Smit1, P W Van Wyk, J P vd Westhuizen
1Department of Dermatology, Medical Faculty, University of the Orange Free State, Bloemfontein, Republic of South Africa.
Abstract:
The computer program described enables a rapid calculation of relative molecular masses of proteins from different yeasts and microbial pathogens, by interpolation from a molecular weight calibration curve that comprises stepwise linear regression between the protein bands produced by the internal standards. A similarity matrix can then be produced, taking into account variations between calculated molecular masses caused by small differences in bandwidths and/or positions of specific protein bands. This program has been applied to different Candida species and the similarity data obtained further analysed numerically utilizing CLUSTAN II on a SPERRY 1100 multi-processor.