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Related Experiment Video

Updated: May 21, 2026

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications
09:56

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Published on: December 8, 2015

Antimicrobial biocompatible bioscaffolds for orthopaedic implants.

Ammar T Qureshi1, Lekeith Terrell, W Todd Monroe

  • 1Department of Agricultural and Biological Engineering, Louisiana State University and Agricultural Center, Baton Rouge, LA, USA.

Journal of Tissue Engineering and Regenerative Medicine
|June 16, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed novel antimicrobial bioscaffolds using poly-l-lactic acid (PLLA) and poly-ethyleneglycol (PEG) with silver nanoparticles (SNPs). These scaffolds effectively reduce bacterial growth and support human stem cell proliferation for tissue engineering.

Keywords:
PLLA-PEGantimicrobialhuman adipose stem cellsnon-cytotoxic and tissue engineeringscaffolds

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Nanotechnology

Background:

  • Orthopaedic trauma and joint replacements necessitate bone grafts, with infection rates reaching 66% and mortality up to 2% post-internal fixation.
  • Osteomyelitis and associated mortality pose significant challenges in orthopaedic trauma management.
  • Need for advanced biomaterials that are antimicrobial, biocompatible, and promote tissue regeneration.

Purpose of the Study:

  • To synthesize and characterize antimicrobial and biocompatible poly-l-lactic acid (PLLA) and poly-ethyleneglycol (PEG) bioscaffolds.
  • To incorporate silver nanoparticles (SNPs) for controlled release, aiming to reduce bacterial growth and support human adipose-derived stem cell (hASC) proliferation.
  • To evaluate the efficacy and safety of these novel bioscaffolds for tissue-engineering applications.

Main Methods:

  • Fabrication of PLLA-PEG bioscaffolds with varying concentrations of SNPs.
  • Characterization using scanning transmission electron microscopy (SEM) to analyze scaffold architecture.
  • Antimicrobial efficacy testing against Staphylococcus aureus and Escherichia coli using a modified ISO 22196 standard.
  • In vitro cell culture studies with hASCs to assess cell viability and proliferation using AlamarBlue® and MTT assays.
  • Quantification of leached silver using inductively coupled plasma optical emission spectroscopy (ICP-OES).

Main Results:

  • SEM revealed dendritic porous channels in bioscaffolds with increasing SNP concentrations.
  • Bioscaffolds demonstrated significant antimicrobial activity, reducing viable Staphylococcus aureus and Escherichia coli by 98.85% and 99.9% respectively at 2000 ppm SNPs.
  • In vitro studies confirmed hASC growth and proliferation on the antimicrobial bioscaffolds.
  • Leached silver was found to be non-cytotoxic to hASCs, as confirmed by MTT assay.

Conclusions:

  • The developed PLLA-PEG bioscaffolds with SNPs are effective antimicrobial agents against common orthopaedic pathogens.
  • These bioscaffolds support human adipose-derived stem cell viability and proliferation, indicating suitability for tissue engineering.
  • The controlled degradation and silver release profile of these bioscaffolds offer a promising solution for preventing infections in bone grafting and joint replacement surgeries.