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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 21, 2026

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
06:28

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Published on: January 25, 2017

Engineered viral nanoparticles for flow cytometry and fluorescence microscopy applications.

Kelly L Robertson1, Jinny L Liu

  • 1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA. kelly.robertson@nrl.navy.mil

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
|June 16, 2012
PubMed
Summary

Viral nanoparticles (VNPs) offer unique biological advantages for biomedical applications. Flow cytometry and fluorescence microscopy are key techniques for characterizing VNP properties, enabling targeted delivery and imaging.

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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

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Area of Science:

  • Biotechnology and Biomedical Sciences
  • Nanotechnology
  • Molecular Biology

Background:

  • Viral nanoparticles (VNPs) are biologically derived materials with significant potential in medicine and biotechnology.
  • Their inherent properties make them suitable for various applications, including drug delivery and diagnostics.
  • Characterization of VNPs is crucial for understanding their behavior and efficacy.

Purpose of the Study:

  • To highlight the utility of viral nanoparticles (VNPs) as platforms in biotechnology and biomedical fields.
  • To emphasize the importance of fluorescence microscopy and flow cytometry in characterizing VNPs.
  • To demonstrate how these techniques inform VNP applications in targeting, imaging, and cargo delivery.

Main Methods:

  • Utilizing fluorescence microscopy for direct visualization of VNPs on cell surfaces and within membranes.
  • Employing flow cytometry for quantitative analysis of VNP uptake and targeting specificity.
  • Characterizing VNPs labeled with fluorescent reporters.

Main Results:

  • Fluorescence microscopy enables detailed observation of VNP localization within cells.
  • Flow cytometry provides statistical data on VNP cellular uptake and specificity.
  • These methods are essential for assessing VNP behavior and potential.

Conclusions:

  • Viral nanoparticles are versatile platforms for biomedical applications.
  • Fluorescence microscopy and flow cytometry are indispensable tools for VNP characterization.
  • Understanding VNP properties through these techniques facilitates their use in targeted therapies and diagnostics.