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Related Concept Videos

Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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Published on: September 22, 2011

High-throughput SNP-based authentication of human cell lines.

Felipe Castro1, Wilhelm G Dirks, Silke Fähnrich

  • 1Department of Genome Modifications and Carcinogenesis, F020, Research Program Infection and Cancer, German Cancer Research Center, DKFZ, Heidelberg, Germany.

International Journal of Cancer
|June 16, 2012
PubMed
Summary
This summary is machine-generated.

Multiplex cell authentication (MCA) using single nucleotide polymorphism profiling offers a robust alternative to short tandem repeat (STR) profiling for human cell line authentication, especially for mismatch repair-deficient cell lines.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • False cell lines pose a significant challenge in biological research.
  • Short tandem repeat (STR) profiling is the standard for cell line authentication.
  • Microsatellite instability in mismatch repair (MMR)-deficient cell lines can lead to misclassification by STR profiling.

Purpose of the Study:

  • To develop and evaluate a novel multiplex cell authentication (MCA) assay for human cell line authentication.
  • To compare the performance of MCA with traditional STR profiling, particularly for MMR-deficient cell lines.

Main Methods:

  • Development of a 24-plex single nucleotide polymorphism (SNP) profiling assay on the Luminex platform.
  • Evaluation of the MCA assay using 436 human cell lines from the German Collection of Microorganisms and Cell Cultures.
  • Comparative analysis with established eight-loci STR profiling.

Main Results:

  • High concordance between MCA and STR profiling was observed.
  • MCA demonstrated similar average matching probabilities to STR profiling (~1 × 10^-9 vs ~1 × 10^-8).
  • MCA effectively detected low levels of contaminating cells (<3%) and showed higher robustness with MMR-deficient cell lines.

Conclusions:

  • MCA serves as a valuable complement to routine cell line authentication methods.
  • MCA is a robust alternative to STR profiling, particularly for microsatellite instability (MSI) cell lines.
  • The MCA assay enhances the reliability of human cell line authentication.