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Related Concept Videos

Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...
Crossing Over01:34

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Related Experiment Video

Updated: May 21, 2026

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
06:18

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

Defining and detecting crossover-interference mutants in yeast.

Frank Stahl1

  • 1Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America. fstahl@uoregon.edu

Plos One
|June 16, 2012
PubMed
Summary
This summary is machine-generated.

Analyzing crossover interference is complex due to two crossover types. This study formalizes interference analysis, accounting for crossover frequencies in yeast mutants.

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Area of Science:

  • Genetics and Molecular Biology
  • Yeast genetics
  • Meiosis and recombination

Background:

  • Crossover interference, a phenomenon where one crossover influences the likelihood of another, is crucial for accurate genetic mapping.
  • Traditional interference analysis is complicated by the presence of both interfering and noninterfering crossover types.
  • The relative frequencies of these crossover types can confound the interpretation of interference strength.

Purpose of the Study:

  • To formalize the relationship between crossover interference, crossover frequencies, and interference indicators.
  • To assess the possibilities and limitations of classical interference analysis in the presence of multiple crossover types.
  • To investigate interference patterns in Saccharomyces cerevisiae, including wild-type and specific mutants.

Main Methods:

  • Utilizing meiotic tetrad data from Saccharomyces cerevisiae.
  • Analyzing data from wild-type yeast strains.
  • Examining data from mlh1 and ndj1 mutant strains, known to affect recombination and repair pathways.

Main Results:

  • Developed a formalized model to disentangle the effects of interference strength and crossover frequencies.
  • Demonstrated that traditional interference indicators are influenced by both factors.
  • Identified specific patterns of interference in wild-type and mutant yeast strains, highlighting limitations of classical methods.

Conclusions:

  • Classical interference analysis requires careful consideration of multiple crossover types and their frequencies.
  • The formalized approach provides a more accurate assessment of crossover interference.
  • Mutations in MLH1 and NDJ1 impact interference patterns in Saccharomyces cerevisiae, offering insights into meiotic recombination regulation.