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Expression of TRPC6 in renal cortex and hippocampus of mouse during postnatal development
Pengjuan Xu1, Jing Xu, Zhigui Li
1College of Medicine, Nankai University, Tianjin, China.
Insights
Transient Receptor Potential Canonical 6 (TRPC6) expression changes during mouse development. TRPC6 decreased in the kidney cortex but increased in the hippocampus, suggesting roles in organ development.
Area of Science:
- Neuroscience
- Nephrology
- Developmental Biology
Background:
- Transient Receptor Potential Canonical 6 (TRPC6) is implicated in various diseases and has diverse functions in the brain and kidney.
- Understanding TRPC6's role during early development is crucial for comprehending organogenesis and potential disease mechanisms.
Purpose of the Study:
- To investigate the expression patterns and functional significance of TRPC6 in the mouse renal cortex and hippocampus during early postnatal development.
Main Methods:
- Immunohistochemistry and Western blotting were employed to analyze TRPC6 expression.
- Expression levels were assessed in mouse kidney and hippocampus at multiple postnatal days (P1-P49).
Main Results:
- TRPC6 expression significantly increased in the developing mouse hippocampus between postnatal days 7 and 14.
- TRPC6 was detected in the glomerulus and tubules of the kidney, with expression decreasing during postnatal maturation of the renal cortex.
- TRPC6 expression showed dynamic changes, decreasing in the kidney cortex and increasing in the hippocampus with postnatal development.
Conclusions:
- Developmental changes in TRPC6 expression are essential for proper postnatal kidney cortex development.
- TRPC6 plays a critical role in hippocampal development, influencing neurogenesis.
- These findings provide foundational knowledge for understanding mouse nephrogenesis and neurogenesis, offering clinical insights.
Abstract:
TRPC6, a member of the TRPC family, attracts much attention from the public because of its relationship with the disease. In both the brain and kidney, TRPC6 serves a variety of functions. The aim of the present study was to observe the expression and effects of TRPC6 in renal cortex and hippocampus during early postnatal development of the mouse. In the present study, immunohistochemistry and Western blotting were used to detect the expression of TRPC6 in the mouse kidney and hippocampus of postnatal day 1, 3, 5, 7, 14, 21, 28 and 49 (P1, P3, P5, P7, P14, P21, P28 and P49). Results showed that the expression of TRPC6 was increased in the mouse hippocampus, and there was a significant increase between P7 and P14 during the postnatal development. Meanwhile, the expression of TRPC6 was also detected in glomerulus and tubules, and a decreased expression was found during postnatal maturation of mouse renal cortex. From these in vivo experiments, we concluded that the expression of TRPC6 was active in the developing mouse kidney cortex, and followed a loss of expression with the development of kidney. Meanwhile, an increased expression was found in the hippocampus with the development. Together, these data suggested that the developmental changes in TRPC6 expression might be required for proper postnatal kidney cortex development, and played a critical role in the hippocampus during development, which formed the basis for understanding the nephrogenesis and neurogenesis in mice and provided a practically useful knowledge to the clinical and related research.
