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The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Published on: October 5, 2016
A reference linkage map for Eucalyptus.
Corey J Hudson1, Jules S Freeman, Anand R K Kullan
1School of Plant Science and CRC for Forestry, University of Tasmania, Private Bag 55 Hobart, Tasmania, 7001, Australia. cjhudson@utas.edu.au
BMC Genomics
|June 19, 2012
Summary
A new composite genetic linkage map was created for eucalypts by merging data from seven studies. This highly saturated map, containing 4101 markers, links directly to the Eucalyptus grandis genome sequence, advancing eucalypt research.
Area of Science:
- Plant genetics
- Forestry
- Genomics
Background:
- Genetic linkage maps are crucial for plant research, aiding in QTL mapping, comparative genomics, and gene cloning.
- Eucalypts are ecologically dominant and economically important trees, with the recent release of the E. grandis genome sequence opening new research avenues.
- A robust, sequence-based reference linkage map is essential for leveraging genomic resources in eucalypts.
Purpose of the Study:
- To develop a single, comprehensive reference genetic linkage map for the Eucalyptus genus.
- To integrate data from multiple independent mapping experiments to create a high-density map.
- To facilitate genomic research and applications in eucalypts by linking genetic and physical maps.
Main Methods:
- A composite genetic linkage map was constructed by merging data from seven independent mapping experiments involving 1950 individuals.
- Sequence-based molecular markers, including Diversity Arrays Technology (DArT) and microsatellites, were utilized.
- A marker-merging approach was employed to integrate the datasets.
Main Results:
- The composite map spans 1107 cM and comprises 4101 markers (3880 DArT, 213 microsatellite, 8 candidate genes).
- After accounting for redundant markers, the map contains 3634 unique loci with an average interval of 0.31 cM.
- Eighty-one DArT markers mapped to multiple linkage groups, contributing to 4191 total map positions.
Conclusions:
- This composite map is the most saturated linkage map developed to date for Eucalyptus.
- The inclusion of sequenced DArT markers provides a direct bridge to the E. grandis genome sequence.
- The map will serve as a vital reference for future genetic and genomic studies in Eucalyptus.