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Related Experiment Video

Updated: May 21, 2026

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

Solvation dynamics under a microscope: single giant lipid vesicle.

Supratik Sen Mojumdar1, Shirsendu Ghosh, Tridib Mondal

  • 1Physical Chemistry Department, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, India.

Langmuir : the ACS Journal of Surfaces and Colloids
|June 19, 2012
PubMed
Summary

Picosecond spectroscopy reveals heterogeneous solvation dynamics and local friction within a single giant lipid vesicle using coumarin 153 (C153) as a probe. Confocal microscopy effectively maps these variations inside the vesicle.

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Last Updated: May 21, 2026

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Area of Science:

  • Physical Chemistry
  • Biophysical Chemistry
  • Materials Science

Background:

  • Understanding molecular behavior within complex biological environments like lipid vesicles is crucial for cell membrane studies.
  • Solvation dynamics and local friction influence chemical reaction rates and molecular interactions.
  • Giant lipid vesicles serve as model systems for cellular membranes, offering insights into their compartmentalized nature.

Purpose of the Study:

  • To investigate the solvation dynamics and diffusion of coumarin 153 (C153) within a single giant lipid vesicle (1,2-dilauroyl-sn-glycero-3-phosphocholine, DLPC).
  • To determine if confocal microscopy can spatially resolve heterogeneity in local friction and solvation within a lipid vesicle.
  • To correlate probe diffusion with solvation dynamics to understand the microenvironment of the vesicle.

Main Methods:

  • Employed picosecond spectroscopy combined with confocal microscopy to probe coumarin 153 (C153) inside a 20 μm DLPC giant lipid vesicle.
  • Utilized fluorescence correlation spectroscopy (FCS) to measure the time-dependent diffusion coefficient (D(t)) of C153.
  • Analyzed the distribution of D(t) to infer microenvironmental heterogeneity and local friction.

Main Results:

  • Observed a wide distribution of diffusion coefficients (D(t)) for C153 within the vesicle, ranging from ~3 to 21 μm(2) s(-1), significantly lower than in bulk water.
  • The heterogeneous diffusion suggests that C153 probes different regions within the vesicle, likely the membrane interface rather than the aqueous core.
  • Measured solvation times for C153 varied between 750 to 1200 ps, indicating spatially dependent solvation dynamics.

Conclusions:

  • Confocal picosecond spectroscopy can effectively resolve spatial heterogeneity in local friction and solvation dynamics within a single giant lipid vesicle.
  • The microenvironment inside the DLPC vesicle is highly heterogeneous, impacting both probe diffusion and solvation.
  • The results highlight the utility of advanced spectroscopic techniques for studying complex molecular environments in model biological systems.